Journal: EMBO Reports

Article Title: An hepatitis B and D virus infection model using human pluripotent stem cell-derived hepatocytes

doi: 10.1038/s44319-024-00236-0

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit anti-FoxA2 (1:400) was purchased from Cell Signaling, mouse anti-AFP (1:1000) from Sigma-Aldrich, mouse anti-ALB (1:1000) from Cedarlane, mouse anti-CD63 from Santa Cruz Biotechnology (1:400), rabbit anti-ADAR1 from Cell Signaling (1:1000), rabbit anti-LDLR from Abcam (1:200), rabbit anti-LAMP1 (1:200) and rabbit anti-EGFR (1:100) from Cell Signaling and rabbit anti-SCARB1 from Novus Biologicals (1:500).

Techniques: Recombinant, Immunofluorescence, Western Blot, Sequencing, Membrane, Knock-Out, Transfection, Reverse Transcription, SYBR Green Assay, Protease Inhibitor, Diagnostic Assay, Software, Microscopy, Imaging

Journal: Experimental Biology and Medicine

Article Title: Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates

doi: 10.1177/15353702221113860

Figure Lengend Snippet: GC-FP-based detection of Nuc protein using MM05 as the capture antibody and R019 as the detection antibody. Positive controls included Nuc protein and R019 antibodies directly immobilized on the GC-FP chip. Negative controls included human IgG and CoV-2 S1S2 (spike) protein. One-way ANOVA followed by Tukey’s multiple comparison test were performed using these data. *** P = 0.0004. **** P < 0.0001.

Article Snippet: Nucleocapsid (Nuc) protein, the S1 fragment of the spike protein (S1), the extracellular domain of the spike protein (S1S2), human serum albumin (HSA), MM05 monoclonal mouse anti-Nuc antibody (MM05), MM08 monoclonal mouse anti-Nuc antibody (MM08), R019 monoclonal rabbit anti-Nuc antibody (R019), and R004 monoclonal rabbit anti-Nuc antibody (R004) were all obtained from Sino Biological (Wayne, PA), Inc.

Techniques:

Journal: Experimental Biology and Medicine

Article Title: Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates

doi: 10.1177/15353702221113860

Figure Lengend Snippet: Direct SARS-CoV-2 Nuc antigen detection via GC-FP for the capture/detection antibody pairs of MM05/R004 and R004/MM05. One-way ANOVA and Tukey’s multiple comparison test showed significant differences between the capture/detection antibody pairs and negative controls. * P < 0.05. **** P < 0.0001.

Article Snippet: Nucleocapsid (Nuc) protein, the S1 fragment of the spike protein (S1), the extracellular domain of the spike protein (S1S2), human serum albumin (HSA), MM05 monoclonal mouse anti-Nuc antibody (MM05), MM08 monoclonal mouse anti-Nuc antibody (MM08), R019 monoclonal rabbit anti-Nuc antibody (R019), and R004 monoclonal rabbit anti-Nuc antibody (R004) were all obtained from Sino Biological (Wayne, PA), Inc.

Techniques:

Journal: Experimental Biology and Medicine

Article Title: Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates

doi: 10.1177/15353702221113860

Figure Lengend Snippet: Evaluation of capture and detection antibody pairs on V22 chips for direct detection of CoV-2 Nuc protein.

Article Snippet: Nucleocapsid (Nuc) protein, the S1 fragment of the spike protein (S1), the extracellular domain of the spike protein (S1S2), human serum albumin (HSA), MM05 monoclonal mouse anti-Nuc antibody (MM05), MM08 monoclonal mouse anti-Nuc antibody (MM08), R019 monoclonal rabbit anti-Nuc antibody (R019), and R004 monoclonal rabbit anti-Nuc antibody (R004) were all obtained from Sino Biological (Wayne, PA), Inc.

Techniques: Negative Control